幹細胞マーカーとしてのアルカリホスファターゼ

起源の異なる幹細胞はそれぞれ異なる増殖条件を必要とし、異なる細胞表面マーカーを発現しますが、下表に示す通りアルカリホスファターゼ(AP)は最も広く使用されている幹細胞マーカーです。StemTAG™アルカリホスファターゼ染色キットは免疫細胞化学染色や活性測定によりES細胞の未分化/分化をモニタリングするための効率的なシステムを提供します。

活性測定キットStemTAG™ Alkaline Phosphatase Activity Assay Kit

比色検出によりアルカリホスファターゼ活性を定量する、便利な96 ウェルフォーマットの活性測定キットです。

使用文献

1. Ito, K. et al. (2020). MicroRNA-204 regulates osteogenic induction in dental follicle cells. J Dent Sci. doi: 10.1016/j.jds.2019.11.004 (#CBA-301).
2. Nam, Y.J. et al. (2020). CRH receptor antagonists from Pulsatilla chinensis prevent CRH-induced premature catagen transition in human hair follicles. J Cosmet Dermatol. doi: 10.1111/jocd.13328 (#CBA-301).
3. Escobar, A. et al. (2019). Mesoporous Titania Coatings with carboxylated Pores for Complexation and slow Delivery of Strontium for osteogenic Induction. Appl Surf Sci. doi: 10.1016/j.apsusc.2019.145172 (#CBA-301).
4. Escobar, A. et al. (2019). Strontium Titanate (SrTiO3) Mesoporous Coatings for Enhanced Strontium Delivery and Osseointegration on Bone Implants. Adv. Eng. Mater. doi:10.1002/adem.201801210 (#CBA-301).
5. Li, J. et al. (2019). Osteogenic capacity and cytotherapeutic potential of periodontal ligament cells for periodontal regeneration in vitro and in vivo. PeerJ. 7:e6589. doi: 10.7717/peerj.6589 (#CBA-301).
6. Escobar, A. et al. (2019). Antibacterial Mesoporous Titania Films with Embedded Gentamicin and Surface Modified with Bone Morphogenetic Protein 2 to Promote Osseointegration in Bone Implants. Advanced Materials Interfaces. 1801648. doi:10.1002/admi.201801648 (#CBA-301).
7. Cheng, J. et al. (2019). Stilbene glycoside protects osteoblasts against oxidative damage via Nrf2/HO-1 and NF-κB signaling pathways. Arch Med Sci. 15(1):196-203. doi: 10.5114/aoms.2018.79937 (#CBA-301).
8. Guo, Y.C. et al. (2018). Ubiquitin-specific protease USP34 controls osteogenic differentiation and bone formation by regulating BMP2 signaling. EMBO J. 37(20). pii: e99398. doi: 10.15252/embj.201899398 (#CBA-301).
9. Xiong, S. et al. (2018). Immunization with Na+/K+ ATPase DR peptide prevents bone loss in an ovariectomized rat osteoporosis model. Biochem Pharmacol. 156:281-290. doi: 10.1016/j.bcp.2018.08.024 (#CBA-301).
10. Camp, E. et al. (2018). miRNA-376c-3p Mediates TWIST-1 Inhibition of Bone Marrow-Derived Stromal Cell Osteogenesis and Can Reduce Aberrant Bone Formation of TWIST-1 Haploinsufficient Calvarial Cells. Stem Cells Dev. 27(23):1621-1633. doi: 10.1089/scd.2018.0083 (#CBA-301).
11. Yang, F. et al. (2018). Fatty acids modulate the expression levels of key proteins for cholesterol absorption in Caco-2 monolayer. Lipids Health Dis. 17(1):32. doi: 10.1186/s12944-018-0675-y(#CBA-301).
12. Abueva, C. D. G. et al. (2018). Multi-channel biphasic calcium phosphate granules as cell carrier capable of supporting osteogenic priming of mesenchymal stem cells. Materials & Design. 141:142–149. doi:10.1016/j.matdes.2017.12.040 (#CBA-301).
13. Imai, K. et al. (2017).  Influence of Fluoride Contamination on Titanium Surface on Cell Viability and Cell Differentiation of ES-D3 Cells. J Oral Tissue Engin. 15(1):35-40. doi: 10.11223/jarde.15.35 (#CBA-301).
14. Imai, K. et al. (2017). Study of ES Cell Differentiation using Three-dimensional Culture with Silica Fiber. Nano Biomedicine. 9(2):55-60. doi: 10.11344/nano.9.55 (#CBA-301).
15. Kamiya, N. et al. (2017). Targeted disruption of NF1 in osteocyte increases FGF23 and osteoid with osteomalacia-like bone phenotype. J Bone Miner Res. doi: 10.1002/jbmr.3155 (#CBA-301).
16. Jin, H. et al. (2016). Increased activity of TNAP compensates for reduced adenosine production and promotes ectopic calcification in the genetic disease ACDC. Sci. Signal. 9:ra121 (#CBA-301).
17. Lee, H. Y. et al. (2016). Porcine placenta hydrolysates enhance osteoblast differentiation through their antioxidant activity and effects on ER stress. BMC Complement Altern Med. doi:10.1186/s12906-016-1274-y(#CBA-301).
18. Choi, H. Y. et al. (2016). Efficient mRNA delivery with graphene oxide-polyethylenimine for generation of footprint-free human induced pluripotent stem cells. J Control Release. 235:222-235 (#CBA-301).
19. Pengjam, Y. et al. (2016). Anthraquinone glycoside aloin induces osteogenic initiation of MC3T3-E1 cells: Involvement of MAPK mediated wnt and bmp signaling. Biomol Ther. 24:123-131 (#CBA-301).
20. Yue, Y. et al. (2015). Safe and bodywide muscle transduction in young adult Duchenne muscular dystrophy dogs with adeno-associated virus. Hum Mol Genet. doi:10.1093/hmg/ddv310 (#CBA-301).
21. Pino-Barrio, M. J. et al. (2015). V-myc immortalizes human neural stem cells in the absence of pluripotency-associated traits. PLoS One. 10:e0118499 (#CBA-301).
22. Pan, X. et al. (2015). AAV-8 is more efficient than AAV-9 in transducing neonatal dog heart. Hum Gene TherMethods. doi:10.1089/hgtb.2014.128 (#CBA-301).
23. Salem, O. et al. (2014). Naproxen affects osteogenesis of human mesenchymal stem cells via regulation of Indian hedgehog signaling molecules. Arthritis Res Ther. 16:R152 (#CBA-301).
24. Guo, L. et al. (2014). Effects of erythropoietin on osteoblast proliferation and function. Clin Exp Med. 14:69-76(#CBA-301).
25. Dong, Y. et al. (2014). NOTCH-mediated maintenance and expansion of human bone marrow stromal/stem cells: a technology designed for orthopedic regenerative medicine. Stem Cells Transl Med. 3:1456-1466 (#CBA-301).
26. Dixon, J.E. et al. (2014). Combined hydrogels that switch human pluripotent stem cells from self-renewal to differentiation. PNAS 111:5580-5585 (#CBA-301).
27. Moussalli, M.J. et al. (2011). Mechanistic Contribution of Ubiquitous 15-Lipoxygenase-1 Expression Loss in Cancer Cells to Terminal Cell Differentiation Evasion. Cancer Prevention Research 4:1961-1972. (#CBA-301).

細胞染色キットStemTAG™ Alkaline Phosphatase Staining Kit

免疫細胞化学染色によりアルカリホスファターゼ活性をモニタリングするための試薬です。

使用文献

1. Zhang, Y. et al. (2020). SENP3 Suppresses Osteoclastogenesis by De-conjugating SUMO2/3 from IRF8 in Bone Marrow-Derived Monocytes. Cell Rep. 30(6):1951-1963.e4. doi: 10.1016/j.celrep.2020.01.036 (#CBA-306).
2. Li, N. et al. (2020). miR-144-3p suppresses osteogenic differentiation of bone marrow mesenchymal stem cells from patients with aplastic anemia through repression of TET2. Mol Ther Nucleic Acids. doi: 10.1016/j.omtn.2019.12.017 (#CBA-306).
3. Liao, W. et al. (2019). BMSCs-derived Exosomes Carrying MicroRNA-122-5p Promote Progression of Osteoblasts in Osteonecrosis of the Femoral Head. Clin Sci (Lond). pii: CS20181064. doi: 10.1042/CS20181064 (#CBA-300).
4. Clarke, D. et al. (2018). Genetically Corrected iPSC-Derived Neural Stem Cell Grafts Deliver Enzyme Replacement to Affect CNS Disease in Sanfilippo B Mice. Mol Ther Methods Clin Dev. 10:113-127. doi: 10.1016/j.omtm.2018.06.005 (#CBA-300).
5. Vitali, M.S. et al. (2017). Use of the spectrophotometric color method for the determination of the age of skin lesions on the pig carcass and its relationship with gene expression and histological and histochemical parameters. J. Anim. Sci. 95(9):3873-3884. doi: 10.2527/jas2017.1813 (#CBA-300).
6. Khan, M.I. et al. (2016). Comparative gene expression profiling of primary and metastatic renal cell carcinoma stem cell-like cancer cells. PLoS One 11:e0165718 (#CBA-306).
7. Lee, K. H. et al. (2016). In vitro ectopic behavior of porcine spermatogonial germ cells and testicular somatic cells. Cell Reprogram. doi:10.1089/cell.2015.0070 (#CBA-300).
8. Lee, K. H. et al. (2015). Subculture of germ cell-derived colonies with GATA4-positive feeder cells from neonatal pig testes. Stem Cells Int. 6029271 (#CBA-300).
9. Jacinto, F. V. et al.  (2015). The nucleoporin Nup153 regulates embryonic stem cell pluripotency through gene silencing. Genes Dev. 29:1224-1238 (#CBA-300).
10. Langlois, T. et al. (2014). TET2 deficiency inhibits mesoderm and hematopoietic differentiation in human embryonic stem cells. Stem Cells. 32:2084-2097 (#CBA-300).
11. Lee, K. H. et al. (2014). Identification and in vitro derivation of spermatogonia in beagle testis. PLoSOne. 9:e109963 (#CBA-300).
12. Manukyan, M. & Singh, P. B. (2014). Epigenome rejuvenation: HP1β mobility as a measure of pluripotent and senescent chromatin ground states. Sci Rep. 4:4789 (#CBA-300).
13. Lee, J. el al. (2010). Ultraviolet A Regulates Adipogenic Differentiation of Human Adipose Tissue-derived Mesenchymal Stem Cells via Up-Regulation of Kruppel-like Factor 2. J. Biol. Chem. 285:32647-32656 (#CBA-300).
14. Izadyar, F. et al. (2008). Generation of multipotent cell lines from a distinct population of male germ line stem cells. Reproduction 135:771-784 (#CBA-300).