TEM of Human Tau-441/2N4R Pre-formed Fibrils Protein (Cat. No. TAU-H5115).TEM of alpha-synuclein preformed fibrils (Cat. No. ALN-H51H4).
TEM of Human Tau-441 K18 Pre-formed Fibrils Protein (Cat. No. TAU-H5146).
セルベースアッセイによるPFF体の凝集確認
Tau PFFs (TAU-H5115)は、HEK293/ヒト タウ-K18 (GFP) 安定細胞株の細胞質内で顕著なタンパク質凝集を誘導しました。
HEK293/Human Tau(GFP) Stable Cell Line were transduced with Human Tau-441 / 2N4R Pre-formed Fibrils Protein, Tag Free (ThT active) (Cat. No. TAU-H5115) and Human Tau-441 / 2N4R Protein, Tag Free (MALS verified) (Cat. No. TAU-H5117) respectively. The fluorescence of GFP-Tau (Green) and DAPI (Blue) were detected by confocal microscope. A. Lipo2000 transduction. B. Lipo2000 and Human Tau-441 / 2N4R Protein, Tag Free (MALS verified) transduction. C. Lipo2000 and Human Tau-441 / 2N4R Pre-formed Fibrils Protein, Tag Free (ThT active) transduction. Scale bars, 50 μm.
Tau PFFs (TAU-H5146)は、HEK293/ヒト タウ (GFP) 安定細胞株の細胞質内で顕著なタンパク質凝集を誘導しました。
HEK293/Human Tau (GFP) Stable Cell Line were transduced with Human Tau-441 K18 Pre-formed Fibrils Protein, His Tag (Cat. No. TAU-H5146) and Human Tau-441 K18 Protein, His Tag respectively. The fluorescence of GFP-Tau (Green) was detected bA. Lipo2000 transduction. B. Lipo2000 and Human Tau-441 K18 Protein, His Tag transduction. C. Lipo2000 and Human Tau-441 K18 Pre-formed Fibrils Protein, His Tag transduction (Routinely tested).
Lipo2000 transduction. B. Lipo2000 and Human Alpha-Synuclein Protein, His Tag transduction. C. Lipo2000 and Human Alpha-Synuclein Pre-formed Fibrils Protein, His Tag (ThT active) transduction. Scale bars, 50 μm.
ThT蛍光アッセイによるPFF体の活性確認
PFF体は、モノマーのみを添加した対照グループよりも早くタンパク質の凝集を誘導しました。
Thioflavin T emission curves show increased fluorescence (correlated to tau aggregation) over time when tau wild-type monomers (Cat. No. TAU-H5117) are combined with tau wild-type Pre-formed Fibrils (Cat. No. TAU-H5115).
Thioflavin T emission curves show increased fluorescence (correlated to tau aggregation) over time when tau K18 monomers are combined with Human Tau-441 K18 Pre-formed Fibrils Protein, His Tag (Cat. No. TAU-H5146) (QC tested). Thioflavin T is a sensitive fluorescence reporter of fibrils formulation that binds to beta sheet-rich structures. The alpha synuclein pre-formed fibrils (Cat. No. ALN-H51H4) is able to induce the aggregation of alpha synuclein monomers (Cat. No. ALN-H52H8) (QC tested).
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ACROBiosystems
ALN-H5115
Human Alpha-Synuclein Pre-formed Fibrils Protein, Tag Free
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